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These are mostly discharged into aquatic ecosystems and transported by currents to diverse areas, first through the rivers, which channel the waste generated in terrestrial sources, into the estuaries, and finally into the oceans. Plastic marine debris are exposed to physical, chemical, and biological stressors, resulting in smaller fragments, known as microplastics. This reality results in severe impacts on global ecosystems, human health, and marine life. There is still a considerable lack of scientific knowledge on both marine litter and microplastics characterization, distribution and sources. It is, therefore, urgent to increase the efforts, especially on transitional ecosystems. Two estuaries from the NW coast of Portugal were considered for the present research. This study aims to evaluate and characterize microplastics in the water column, as well as identify the main sources of such debris, as a long-term research collaborative research. 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