[ { "@id": "https://w3id.org/np/RAZTGowlvcV7tu_Gi1DpIhq3KNCra6KQBgpAdqx7gSMrE/Head", "@graph": [ { "@id": "https://w3id.org/np/RAZTGowlvcV7tu_Gi1DpIhq3KNCra6KQBgpAdqx7gSMrE", "http://www.nanopub.org/nschema#hasAssertion": [ { "@id": "https://w3id.org/np/RAZTGowlvcV7tu_Gi1DpIhq3KNCra6KQBgpAdqx7gSMrE/assertion" } ], "http://www.nanopub.org/nschema#hasProvenance": [ { "@id": "https://w3id.org/np/RAZTGowlvcV7tu_Gi1DpIhq3KNCra6KQBgpAdqx7gSMrE/provenance" } ], "http://www.nanopub.org/nschema#hasPublicationInfo": [ { "@id": "https://w3id.org/np/RAZTGowlvcV7tu_Gi1DpIhq3KNCra6KQBgpAdqx7gSMrE/pubinfo" } ], "@type": [ "http://www.nanopub.org/nschema#Nanopublication" ] } ] }, { "@id": "https://w3id.org/np/RAZTGowlvcV7tu_Gi1DpIhq3KNCra6KQBgpAdqx7gSMrE/pubinfo", "@graph": [ { "@id": "https://orcid.org/0009-0005-7752-8203", "http://xmlns.com/foaf/0.1/name": [ { "@value": "Tiado Kollender" } ] }, { "@id": "https://w3id.org/np/RAZTGowlvcV7tu_Gi1DpIhq3KNCra6KQBgpAdqx7gSMrE", "http://purl.org/dc/terms/created": [ { "@value": "2026-01-11T00:00:00Z", "@type": "http://www.w3.org/2001/XMLSchema#dateTime" } ], "http://purl.org/dc/terms/license": [ { "@id": "https://creativecommons.org/publicdomain/zero/1.0/" } ], "http://purl.org/nanopub/x/wasCreatedFromPubinfoTemplate": [ { "@id": "https://w3id.org/np/RA0J4vUn_dekg-U1kK3AOEt02p9mT2WO03uGxLDec1jLw" }, { "@id": "https://w3id.org/np/RAukAcWHRDlkqxk7H2XNSegc1WnHI569INvNr-xdptDGI" } ], "http://purl.org/pav/createdBy": [ { "@id": "https://orcid.org/0009-0005-7752-8203" } ], "@type": [ "http://purl.org/nanopub/x/ExampleNanopub" ], "http://www.w3.org/2000/01/rdf-schema#comment": [ { "@language": "en", "@value": "AIDA-based assertion about CoLoC-seq innovations compared to existing subcellular transcriptomics methods" } ], "http://www.w3.org/2000/01/rdf-schema#label": [ { "@language": "en", "@value": "Nanopublication: CoLoC-seq design innovations and advantages" } ], "http://www.w3.org/ns/prov#wasAttributedTo": [ { "@id": "https://orcid.org/0009-0005-7752-8203" } ] }, { "@id": "https://w3id.org/np/RAZTGowlvcV7tu_Gi1DpIhq3KNCra6KQBgpAdqx7gSMrE/sig", "http://purl.org/nanopub/x/hasAlgorithm": [ { "@value": "RSA" } ], "http://purl.org/nanopub/x/hasPublicKey": [ { "@value": "MIIBIjANBgkqhkiG9w0BAQEFAAOCAQ8AMIIBCgKCAQEAoSR/rqhnkTPQO8u4l2JQAemJo5cN83rT54nA5NXHUqaeu26bEjT/rsX1PCVBhhJ3cV2uu4wjI0NfcVaKPKmODSf6p579tdOykOnjbWLbxxPMe5DC5eV1ThMHsFsNU/6vCYIfS00E7wre7fnFYz7FAG7v1MZEyWeSXsHgk9jHoqQQDPqup5PHReKp1dd6J0Nht/OfSYnUancKAgIFyPhkTlThsLK5xSNyNEgiTR7pwkP5pGq2AnD6bPw4G3kiPnIpIGZNsaeIOvhn64gwbrtoJVdoun/namw6FQ7StEC5km/0evaBZc4qIextwO/s32Wru77ukblJ0Xi/YzbC0iLuIQIDAQAB" } ], "http://purl.org/nanopub/x/hasSignature": [ { "@value": "AEcAJ6oJVhJfoC+rwTDoB14K+4H9SwrTO4QiGZvk7hmxIEA6aJ+MRZ2x6vfHAjZJj0fwhwgWRxWiVkTpZ9/IdnbTxdY7DvMGrVUBZVHwOMeYTPt+hopVGUA0mzrnXnjYqswfOWTDpLV4JQ1GzlJSj1bGUhmf64yyckFNJvfayyT679qCHYxhcyIuMbSZgovu5mdowThvWf0uP7JjDVhlAdatlx0wtwDPOisrFqqSJQUYee8zObb8+WyLXIjwofxqyFf7owtpjavzBIpEUdHOY/Kstnfrw/rd1+xPFgqtbc1EKk6oDciFi/fV49s+8ZgAGbBqAvd+wi/LkmMQ5bUIlQ==" } ], "http://purl.org/nanopub/x/hasSignatureTarget": [ { "@id": "https://w3id.org/np/RAZTGowlvcV7tu_Gi1DpIhq3KNCra6KQBgpAdqx7gSMrE" } ] } ] }, { "@id": "https://w3id.org/np/RAZTGowlvcV7tu_Gi1DpIhq3KNCra6KQBgpAdqx7gSMrE/assertion", "@graph": [ { "@id": "http://purl.org/coloc-seq/advantage-kinetics-approach", "http://schema.org/advantage": [ { "@language": "en", "@value": "Distinguishes genuinely resident transcripts from slowly degradable contaminants" } ], "http://schema.org/description": [ { "@language": "en", "@value": "Tracks depletion dynamics across gradient instead of single RNase treatment" } ], "http://schema.org/name": [ { "@language": "en", "@value": "Enzymatic Kinetics-Based Approach" } ], "@type": [ "http://schema.org/Thing" ], "http://www.w3.org/2000/01/rdf-schema#comment": [ { "@language": "en", "@value": "Overcomes major limitation of standard fractionation methods" } ] }, { "@id": "http://purl.org/coloc-seq/advantage-mock-control", "http://schema.org/expectedResult": [ { "@language": "en", "@value": "P0 = 0 for all RNAs when membranes are dissolved" } ], "http://schema.org/name": [ { "@language": "en", "@value": "Mock CoLoC-seq Control Experiment" } ], "http://schema.org/purpose": [ { "@language": "en", "@value": "Control for false-positive identifications" }, { "@language": "en", "@value": "Distinguish organellar protection from intrinsic RNase resistance" } ], "@type": [ "http://schema.org/Thing" ], "http://www.w3.org/2000/01/rdf-schema#comment": [ { "@language": "en", "@value": "Essential quality control for validating resident RNA identification" } ] }, { "@id": "http://purl.org/coloc-seq/advantage-no-genetic-modification", "http://schema.org/benefit": [ { "@language": "en", "@value": "Applicable to any cell type or tissue" }, { "@language": "en", "@value": "Broader applicability than proximity labeling methods" }, { "@language": "en", "@value": "Can be implemented in non-model species" } ], "http://schema.org/name": [ { "@language": "en", "@value": "No Genetic Modification Required" } ], "@type": [ "http://schema.org/Thing" ], "http://www.w3.org/2000/01/rdf-schema#comment": [ { "@language": "en", "@value": "Key practical advantage for diverse research applications" } ] }, { "@id": "http://purl.org/coloc-seq/advantage-universal-applicability", "http://schema.org/advantage": [ { "@language": "en", "@value": "Compatible with non-model organisms" }, { "@language": "en", "@value": "No genetic modification required" } ], "http://schema.org/applicableTo": [ { "@language": "en", "@value": "Extracellular vesicles" }, { "@language": "en", "@value": "Mitochondria" }, { "@language": "en", "@value": "Plastids" }, { "@language": "en", "@value": "Vacuolar compartments" }, { "@language": "en", "@value": "Viral particles" } ], "http://schema.org/name": [ { "@language": "en", "@value": "Universal Applicability" } ], "@type": [ "http://schema.org/Thing" ], "http://www.w3.org/2000/01/rdf-schema#comment": [ { "@language": "en", "@value": "Major advantage over proximity labeling techniques" } ] }, { "@id": "https://w3id.org/np/RAZTGowlvcV7tu_Gi1DpIhq3KNCra6KQBgpAdqx7gSMrE", "http://purl.org/nanopub/x/relatesTo": [ { "@id": "https://w3id.org/np/RAMVUimnATifJYJw0VP2zAesw4OK_Gw30p9NgcEU4oVW8" }, { "@id": "https://w3id.org/np/RA_C-L7pLBKSyHDhS2YIFmqfb_-BLGOldcUb8emWvRltA" }, { "@id": "https://w3id.org/np/RAqWgWTy_lLfUrvdgqP0lggMHH_Of_5TgmpDWkpxe16gg" } ], "http://schema.org/about": [ { "@id": "https://doi.org/10.1093/nar/gkac1205" } ], "http://schema.org/text": [ { "@language": "en", "@value": "CoLoC-seq advances subcellular transcriptomics by combining enzymatic kinetics with Mock control experiments to overcome false-positive limitations of conventional fractionation methods and eliminating the requirement for genetic modification." } ], "@type": [ "https://w3id.org/np/RAZTGowlvcV7tu_Gi1DpIhq3KNCra6KQBgpAdqx7gSMrE/o/ntemplate/AIDA-Sentence" ], "http://www.w3.org/2000/01/rdf-schema#seeAlso": [ { "@id": "http://edamontology.org/operation_3680" } ] } ] }, { "@id": "https://w3id.org/np/RAZTGowlvcV7tu_Gi1DpIhq3KNCra6KQBgpAdqx7gSMrE/provenance", "@graph": [ { "@id": "https://doi.org/10.1093/nar/gkac1205", "http://purl.org/ontology/bibo/doi": [ { "@value": "10.1093/nar/gkac1205" } ], "http://schema.org/datePublished": [ { "@value": "2023", "@type": "http://www.w3.org/2001/XMLSchema#gYear" } ], "http://schema.org/isPartOf": [ { "@id": "https://w3id.org/np/RAZTGowlvcV7tu_Gi1DpIhq3KNCra6KQBgpAdqx7gSMrE/__1" } ], "http://schema.org/name": [ { "@language": "en", "@value": "CoLoC-seq probes the global topology of organelle transcriptomes" } ], "@type": [ "http://schema.org/ScholarlyArticle" ] }, { "@id": "https://w3id.org/np/RAZTGowlvcV7tu_Gi1DpIhq3KNCra6KQBgpAdqx7gSMrE/__1", "http://schema.org/name": [ { "@language": "en", "@value": "Nucleic Acids Research" } ], "@type": [ "http://schema.org/Periodical" ] }, { "@id": "https://w3id.org/np/RAZTGowlvcV7tu_Gi1DpIhq3KNCra6KQBgpAdqx7gSMrE/assertion", "http://www.w3.org/ns/prov#wasAttributedTo": [ { "@id": "https://orcid.org/0000-0001-8763-6703" }, { "@id": "https://orcid.org/0000-0002-1481-2996" }, { "@id": "https://orcid.org/0000-0002-9142-7979" } ], "http://www.w3.org/ns/prov#wasDerivedFrom": [ { "@id": "https://doi.org/10.1093/nar/gkac1205" }, { "@id": "https://w3id.org/np/RAMVUimnATifJYJw0VP2zAesw4OK_Gw30p9NgcEU4oVW8" }, { "@id": "https://w3id.org/np/RA_C-L7pLBKSyHDhS2YIFmqfb_-BLGOldcUb8emWvRltA" }, { "@id": "https://w3id.org/np/RAqWgWTy_lLfUrvdgqP0lggMHH_Of_5TgmpDWkpxe16gg" } ] } ] } ]